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<p><b>OBJECTIVE</b>To investigate the distribution of Colton and Diego rare blood group antigens of blood donors in Chinese Xinjiang minorities.</p><p><b>METHODS</b>A multiplex PCR was applied to screen for alleles antigens Diand Coin 1020 randomly selected healthy donors of Chinese Xinjiang minorities by using each 5 samples mixed detection method. The samples in the positive pools were further tested individually. Furthermore, the positive samples, including Di/Diand Co/ Cogenotypes were tested via 2 PCR-SSP assays for high frequency allele Diand Coto get the rare genotypes Di, Co.</p><p><b>RESULTS</b>Among 1020 samples 12 cases with Coallele, 45 cases with Diand 1 case with Diwere identified.</p><p><b>CONCLUSION</b>The frequencies of Diand Coalleles are 2.30% and 0.59%, respectively. The information of rare blood donors obtained from the screening can provide a reference for matched blood transfusion, and further enrich the National Rare Blood Bank of China.</p>
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Objective To investigate stress and bone density distribution changes in the mandible due to the interference fit in titanium dental implants for mandible reconstruction, and study the influence of interference magnitudes on mandibular bone remodeling. Methods Eight interference fit models with titanium implants were established by finite element method. Bone remodeling based on strain energy density (SED) algorithm was employed to investigate changes in stress and bone density of the mandible in the models during 36 months with inference magnitude of 5%, 10%, 15%, 20%, 25%, 30%, 35% and 40%, respectively. Results When the diameter of pilot hole was larger than the internal diameter of titanium screw, the increment of interference magnitude would gradually enhance the screw fixation at the bone titanium dental implant interface. However, once the magnitude of interference exceeded 30%, the increase in interference magnitude might weaken the screw fixation due to the failure and loss of bone. Conclusions The diameter of pilot hole equal to or slightly smaller than the internal diameter of titanium screw is recommended for mandibular reconstruction.
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Objective To provide an optimization method and a basic configuration for the configuration design of mandibular defect-repair implant. Methods A topology optimization computation based on density method was adopted to make topology optimization on reconstruction titanium plate for repairing mandibular defect. Results The effects of volume fraction F and penalization factor p on the optimized configuration were compared and analyzed, respectively, and the optimum parameters were chosen to be F=0.65 and p=3.5. Using this set of parameters, optimized computation was performed on the mandibular defect-repair implant, and the three opening configuration was proved to be the basic optimization configuration. The result also showed that unilateral molar clenching had more significant effect on stress distributions in the implant as compared to incisal clenching. Conclusions A set of parameters suitable for optimizing configuration of mandibular defect-repair implant, as well as the basic configuration of optimized titanium plate were obtained, which could provide references for the design of mandibular defect-repair implant in clinic.
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The brown slime inside pipeline of the polluted mineral waters well was analyzed. It was confirmed that the brown slime was a biofilm formed mainly by growth of bacteria contaminant, using microscope analysis. After treated with HCl, the brown crystals and mycelioid matter presented in slimes disappeared. It was verified these were metal deposits. The samples produced Prussia blue deposits by dropping K 4Fe(CN) 6 and HCl, which proved that the metal deposits were iron compound. Also it was believed the biofilm was produced by growth of iron bacteria, mixed with deposits of iron compound. The iron bacteria have been isolated from the slimes and cultured in the laboratory. The colony on agar plate also produced Prussia blue deposits after treated with K 4Fe(CN) 6 and HCl. The cells form of pure culture was the same as that in the samples by electronmicroscope analysis. Therefore, It indicated that the bacteria on biofilm inside pipeline were iron bacteria mainly.